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KMID : 0043320100330050703
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Archives of Pharmacal Research
2010 Volume.33 No. 5 p.703 ~ p.708
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Isolation and Quantitative Analysis of Peroxynitrite Scavengers from Artemisia princeps var. orientalis
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Park Hee-Juhn
Nugroho Agung
Lee Kang-Ro
Alam Md. Badrul
Choi Jae-Sue
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Abstract
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Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food and a medicinal plant, respectively, in Korea. Here, we identified the in vitro potent peroxynitrite (ONOO-)-scavenging effect (IC50, 0.26 μg/mL) of the components from the EtOAc fraction. Octadecylsilane column chromatography on the EtOAc fraction yielded two caffeoylquinic acid compounds, 3,5-di-O-caffeoyl-muco-quinic acid (1) and methyl 4,5-di-O-caffeoylquinate (2) by NMR spectroscopic data, which have not been reported before from APO. The IC50 values of compounds 1 and 2 were 0.18 ± 0.01 μg/mL and 0.12 ± 0.00 μg/mL, respectively, lower than that of the positive control (L-penicillamine). HPLC data indicated that young APO (1: 30.3 mg/g dried weight, 2: 27.7 mg/g) contained considerably higher quantities of the two caffeoylquinic acids than mature APO (1: 1.77 mg/g dried weight, 2: 4.10 mg/g).
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KEYWORD
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Artemisia princeps var. orientalis, Compositae, Caffeoylquinic acid, Peroxynitrite, HPLC
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